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KMID : 0545120230330081076
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Journal of Microbiology and Biotechnology
2023 Volume.33 No. 8 p.1076 ~ p.1083
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Poly(3-hydroxybutyrate) Degradation by Bacillus infantis sp. Isolated from Soil and Identification of phaZ and bdhA Expressing PHB Depolymerase
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Ajitabh Srivastava
Jin Hye-Ji
Kong You-Jung
Cha Haeng-Geun
Lee Byung-Wook
Yu Kyung-Jae
Yi Byong-Son
Kim Hee-Taek
Joo Jeong-Chan
Yang Yung-Hun
Lee Jong-Bok
Jung Sang-Kyu
Park See-Hyoung
Park Kyung-Moon
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Abstract
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Poly(3-hydroxybutyrate) (PHB) is a biodegradable and biocompatible bioplastic. Effective PHB degradation in nutrient-poor environments is required for industrial and practical applications of PHB. To screen for PHB-degrading strains, PHB double-layer plates were prepared and three new Bacillus infantis species with PHB-degrading ability were isolated from the soil. In addition, phaZ and bdhA of all isolated B. infantis were confirmed using a Bacillus sp. universal primer set and established polymerase chain reaction conditions. To evaluate the effective PHB degradation ability under nutrient-deficient conditions, PHB film degradation was performed in mineral medium, resulting in a PHB degradation rate of 98.71% for B. infantis PD3, which was confirmed in 5 d. Physical changes in the degraded PHB films were analyzed. The decrease in molecular weight due to biodegradation was confirmed using gel permeation chromatography and surface erosion of the PHB film was observed using scanning electron microscopy. To the best of our knowledge, this is the first study on B. infantis showing its excellent PHB degradation ability and is expected to contribute to PHB commercialization and industrial composting.
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KEYWORD
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Poly(3-hydroxybutyrate), biodegradation, Bacillus infantis, PHB depolymerase, phaZ, bdhA
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